Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro
作者： Cuiqin YANG , Yaoguo QIN , Xin SUN , Shu YUAN , Honghui LIN
DOI: 10.15835/NBHA4016566
关键词: Shoot 、 Somatic embryogenesis 、 Explant culture 、 Murashige and Skoog medium 、 Botany 、 Hyperhydricity 、 Subculture (biology) 、 Biology 、 Tissue culture 、 Plantlet
摘要: An efficient protocol was established for Sedum spectabile Boreau propagation. Various leaf parts were used as explants to regenerate plantlets, the stem segments of which cultured shoot proliferation and plantlet multiplication. The results showed that base optimal explant, compared both middle top leaves, formation. highest induction 88.9% observed on MS medium supplemented with 0.6 mg/l TDZ 0.1 NAA. Hyperhydric leaves obtained in primary culture developed first into abnormal somatic embryos 10 days after subculture, then hyperhydric plantlets an additional days. reversed normal when plant growth regulators removed from medium. Further, regeneration root induction. Optimal containing Root mean number all higher auxin-free than auxins.
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