Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response.

摘要: Hyperhydricity is a physiological disorder frequently affecting shoots vegetatively propagated in vitro. Hyperhydric are characterised by translucent aspect due to chlorophyll deficiency, not very developed cell wall and high water content. of Prunus avium was expressed vitro one multiplication cycle replacing the gelling agent agar (normal shoots: NS) gelrite (hyperhydric HS). P. evolving towards hyperhydric state produced higher amounts ethylene, polyamines (PAs) proline, which substances considered as stress markers. A activity glutathione peroxidase (GPX; EC 1.11.1.9), involved organic hydroperoxide elimination, suggested an increased production these compounds HS. The unchanged free fatty acid composition indicated no HS membrane damages compared NS. ploidy level nuclei affected, but bigger size lower percentage during S phase slowing down cycle. results argued for response HS, signs oxidative lipid nucleus were observed. discussion points out paradoxical classical analysis suggests alternative way defense mechanisms involving homeostatic regulation controlled degradation processes maintain integrity vital functions cell.