Flow cytometry estimation of nuclear size and ploidy level of habituated calli of sugar beet
作者： C. Kevers , R. Greimers , T. Franck , B. Bisbis , J. Dommes
关键词:
摘要: A fully habituated (auxin- and cytokinin-independent) self-regenerating (organo-genic) sugar beet cell line (HO) a non-organogenic one (HNO) derived from the former one, were analyzed as to their nuclear size DNA content. Flow cytometry image analysis used cells of certified diploid leaves same strain served controls. The HNO had been shown previously have many characteristics cancerous cells. analyses made on indicated presence only population cycling In HO cells, two populations detected: first content while second contained fold more than population. showed higher also evidence aneuploidy. Thus, size, ploidy level increase together with neoplasic progression culminate in loss organogenic totipotency.
springer.com
sci-hub.se 参考文章(34)
Walter Handro, Cláudio M. Ferreira, Eny I.S. Floh, Chromosomal variability and growth rate in cell suspension cultures of Stevia rebaudiana (Bert.) Bertoni Plant Science. ,vol. 93, pp. 169- 176 ,(1993) , 10.1016/0168-9452(93)90046-3
C. Kevers, B. Bisbis, F. Le Dily, J. P. Billard, C. Huault, Th. Gaspar, Darkness improves growth and delays necrosis in a nonchlorophyllous habituated sugarbeet callus: Biochemical changes In Vitro Cellular & Developmental Biology – Plant. ,vol. 31, pp. 122- 126 ,(1995) , 10.1007/BF02632249
Frederik Dily, Jean-Pierre Billard, Thomas Gaspar, Claude Huault, Disturbed nitrogen metabolism associated with the hyperhydric status of fully habituated callus of sugarbeet Physiologia Plantarum. ,vol. 88, pp. 129- 134 ,(1993) , 10.1111/J.1399-3054.1993.TB01770.X
C. Kevers, M. Coumans, W. Greef, M. Hofinger, Th. Gaspar, Habituation in sugarbeet callus: auxin content, auxin protectors, peroxidase pattern and inhibitors Physiologia Plantarum. ,vol. 51, pp. 281- 286 ,(1981) , 10.1111/J.1399-3054.1981.TB04478.X
W. De Greef, M. Jacobs, In vitro culture of the sugarbeet: Description of a cell line with high regeneration capacity Plant Science Letters. ,vol. 17, pp. 55- 61 ,(1979) , 10.1016/0304-4211(79)90109-3
Cl. Kevers, M. Coumans, W. de Greef, M. Jacobs, TH. Gaspar, Organogenesis in Habituated Sugarbeet Callus: Auxin Content and Protectors, Peroxidase Pattern and Inhibitors Zeitschrift für Pflanzenphysiologie. ,vol. 101, pp. 79- 87 ,(1981) , 10.1016/S0044-328X(81)80064-5
Fernando T. Nicoloso, John Val, Maarten van der Keur, Frank van Iren, Jan W. Kijne, Flow-cytometric cell counting and DNA estimation for the study of plant cell population dynamics Plant Cell Tissue and Organ Culture. ,vol. 39, pp. 251- 259 ,(1994) , 10.1007/BF00035978
Martin J. Michaelson, H. James Price, John R. Ellison, J. Spencer Johnston, COMPARISON OF PLANT DNA CONTENTS DETERMINED BY FEULGEN MICROSPECTROPHOTOMETRY AND LASER FLOW CYTOMETRY American Journal of Botany. ,vol. 78, pp. 183- 188 ,(1991) , 10.1002/J.1537-2197.1991.TB15745.X
Thomas Gaspar, Daniel Hagege, Claire Kevers, Claude Penel, Michele Crevecoeur, Isabelle Engelmann, Hubert Greppin, Jean-Michel Foidart, When plant teratomas turn into cancers in the absence of pathogens Physiologia Plantarum. ,vol. 83, pp. 696- 701 ,(1991) , 10.1111/J.1399-3054.1991.TB02489.X
M. D. Sacristán, G. Melchers, The caryological analysis of plants regenerated from tumorous and other callus cultures of tobacco Molecular Genetics and Genomics. ,vol. 105, pp. 317- 333 ,(1969) , 10.1007/BF00277587