Organogenesis in Habituated Sugarbeet Callus: Auxin Content and Protectors, Peroxidase Pattern and Inhibitors
作者： Cl. Kevers , M. Coumans , W. de Greef , M. Jacobs , TH. Gaspar
DOI: 10.1016/S0044-328X(81)80064-5
关键词: Sephadex 、 Biochemistry 、 Organogenesis 、 Hormone 、 MOPS 、 Biology 、 Callus 、 Auxin 、 Isozyme 、 Peroxidase 、 General Medicine
摘要: Summary A comparison of the soluble, membrane- and cell wall-bound peroxidases an organogenetic a nonorganogenetic habituated sugarbeet callus showed that tissues contained higher level peroxidase activity larger number isoenzymes. Partially (through G-100 Sephadex) purified likewise exhibited auxin-oxidase activities. Organogenetic also were found to contain levels inhibitors auxin protectors (auxin-oxidase inhibitors). GC-MS analysis IAA indicated lower hormone in callus. This presumably leads auxin-cytokinin ratio compatible with induction shoot formation.
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