The cyclic gene Hes1 contributes to diverse differentiation responses of embryonic stem cells
作者： T. Kobayashi , H. Mizuno , I. Imayoshi , C. Furusawa , K. Shirahige
DOI: 10.1101/GAD.1823109
关键词: Embryonic stem cell 、 Biology 、 GADD45G 、 Neurosphere 、 Cellular differentiation 、 Notch signaling pathway 、 Cell culture 、 Stem cell 、 HES1 、 Cell biology
摘要: Stem cells do not all respond the same way, but mechanisms underlying this heterogeneity are well understood. Here, we found that expression of Hes1 and its downstream genes oscillate in mouse embryonic stem (ES) cells. Those expressing low high levels tended to differentiate into neural mesodermal cells, respectively. Furthermore, inactivation facilitated differentiation more uniformly at earlier time. Thus, Hes1-null ES display less both timing fate choice, suggesting cyclic gene contributes heterogeneous responses even under environmental conditions.
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