MgCHA particles dispersion in porous PCL scaffolds: in vitro mineralization and in vivo bone formation.
作者： Vincenzo Guarino , Silvia Scaglione , Monica Sandri , Marco A. Alvarez-Perez , Anna Tampieri
DOI: 10.1002/TERM.1521
关键词: Scaffold 、 Bone marrow 、 In vitro 、 Lamellar structure 、 Mineralization (biology) 、 Biomedical engineering 、 Biophysics 、 Bone regeneration 、 Mature Bone 、 In vivo 、 Chemistry
摘要: In this work, we focus on the in vitro and vivo response of composite scaffolds obtained by incorporating Mg,CO3-doped hydroxyapatite (HA) particles poly(e-caprolactone) (PCL) porous matrices. After a complete analysis chemical physical properties synthesized (i.e. SEM/EDS, DSC, XRD FTIR), demonstrate that Mg,CO3 doping influences surface wettability with implications upon cell–material interaction new bone formation mechanisms. particular, ion substitution apatite crystals positively early cellular human mesenchymal stem cells (hMSCs), i.e. adhesion proliferation, promotes an extensive mineralization scaffold osteogenic medium, thus conforming to more faithful reproduction native environment than undoped HA particles, used as control PCL Furthermore, support inducing neo-bone 2 months post-implantation, abundant mature tissue at sixth month, lamellar structure completely formed marrow. Together, these results indicate Mg2+ CO32– enhances properties, providing right signals combine morphological requirements pore size, shape interconnectivity) drive scaffold-aided regeneration. Copyright © 2012 John Wiley & Sons, Ltd.
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