Fine Oral Filaments in Paramecium: a Biochemical and Immunological Analysis
作者： JEAN-CLAUDE CLEROTa , FRANCINE IFTODEa , KARINE BUDINa , RACHEL JEANMAIRE-WOLFa , GERARD COFFEb
DOI: 10.1111/J.1550-7408.2001.TB00310.X
关键词: Biology 、 Cell biology 、 Immunogold labelling 、 Epitope 、 Immunofluorescence 、 Oral apparatus 、 Paramecium 、 Monoclonal antibody 、 Cytoskeleton 、 Antigen
摘要: In Paramecium, several kinds of the oral networks fine filaments are defined at ultrastructural level. Using sodium chloride-treated apparatus Paramecium as an antigen to produce monoclonal antibodies, we have begun identify proteins constituting these networks. Immunoblotting showed that all positive antibodies were directed against three bands (70-, 75-and 83-kD), which corresponded quantitatively minor components antigen; there was no antibody specific for major (58- and 62-kD). Immunolocalization with four one or supporting area; a decoration basal bodies outer lattice also observed on cortex. Immunofluorescence interphase cells suggested colocalized left side apparatus, whereas only 70-kD band detected right side. During division, antigens different stages after body assembly. The cross-reacted tetrins, filament-forming in Tetrahymena, demonstrating tetrin-related somatic cytoskeleton Paramecium.
sci-hub.se 参考文章(25)
Francine Iftode, Anne Fleury, André Adoutte, Development of surface pattern during division in Paramecium European Journal of Protistology. ,vol. 33, pp. 145- 167 ,(1997) , 10.1016/S0932-4739(97)80032-6
Jean Grain, The Cytoskeleton in Protists: Nature, Structure, and Functions International Review of Cytology-a Survey of Cell Biology. ,vol. 104, pp. 153- 249 ,(1986) , 10.1016/S0074-7696(08)61926-9
Norman E. Williams, Jerry E. Honts, Chapter 42 Isolation and Fractionation of the Tetrahymena Cytoskeleton and Oral Apparatus Methods in Cell Biology. ,vol. 47, pp. 301- 306 ,(1995) , 10.1016/S0091-679X(08)60823-7
Michel Bornens, Guy Keryer, Francine Iftode, Identification of proteins associated with microtubule-organizing centres and filaments in the oral apparatus of the ciliate Paramecium tetraurelia Journal of Cell Science. ,vol. 97, pp. 553- 563 ,(1990) , 10.1242/JCS.97.3.553
G. Coffe, A. Adoutte, H. Guyader, J. Darmanaden-Delorme, J. C. Clerot, R. Jeanmaire-Wolf, P. Nahon, Identification of the epiplasmins, a new set of cortical proteins of the membrane cytoskeleton in Paramecium Journal of Cell Science. ,vol. 104, pp. 975- 990 ,(1993) , 10.1242/JCS.104.4.975
J L Salisbury, A Baron, B Surek, M Melkonian, Striated Flagellar Roots" Isolation and Partial Characterization of a Calcium-modulated Contractile Organelle Journal of Cell Biology. ,vol. 99, pp. 962- 970 ,(1984) , 10.1083/JCB.99.3.962
SINA M. ADL, JAMES D. BERGER, Commitment to Division in Ciliate Cell Cycles Journal of Eukaryotic Microbiology. ,vol. 43, pp. 77- 86 ,(1996) , 10.1111/J.1550-7408.1996.TB04484.X
Linda Sperling, Guy Keryer, Françoise Ruiz, Janine Beisson, Cortical morphogenesis in Paramecium: a transcellular wave of protein phosphorylation involved in ciliary rootlet disassembly. Developmental Biology. ,vol. 148, pp. 205- 218 ,(1991) , 10.1016/0012-1606(91)90330-6
M Schliwa, J van Blerkom, Structural interaction of cytoskeletal components Journal of Cell Biology. ,vol. 90, pp. 222- 235 ,(1981) , 10.1083/JCB.90.1.222
Norman E. Williams, Jerry E. Honts, Ron W. Graeff, Oral filament proteins and their regulation in Tetrahymena pyriformis Experimental Cell Research. ,vol. 164, pp. 295- 310 ,(1986) , 10.1016/0014-4827(86)90030-3