Image registration of ex-vivo MRI to sparsely sectioned histology of hippocampal and neocortical temporal lobe specimens.

摘要: Abstract Intractable or drug-resistant epilepsy occurs in up to 30% of patients, with many these patients undergoing surgical excision the affected brain region achieve seizure control. Recent magnetic resonance imaging (MRI) sequences and analysis techniques have potential detect abnormalities not identified diagnostic MRI protocols. Prospective studies involving pre-operative collection surgically-resected tissue provide a unique opportunity for verification tuning image techniques, since direct comparison can be made against histopathology, lead better prediction outcomes potentially less invasive procedures. To carry out histology comparison, spatial correspondence between MR images must found. Towards this goal, novel pipeline is presented here bringing ex-vivo temporal lobe specimens digital into correspondence. The sparsely-sectioned represent challenge 3D reconstruction which we address combined 2D registration algorithm that alternates slice-based volume-based MRI. We evaluated our method on resected from anterior lobectomy (N = 7) found mean target error 0.76 ± 0.66 0.98 ± 0.60 mm hippocampal neocortical respectively. This work allows spatially-local post-operative paves way eventual correlation techniques.