Latent membrane protein of Epstein-Barr virus induces cellular phenotypes independently of expression of Bcl-2.

摘要: Abstract The stable expression of the Epstein-Barr virus (EBV) latent membrane protein (LMP) in certain EBV-negative Burkitt's lymphoma cell lines correlates with an increased oncogene Bcl-2 (S. Henderson, M. Rowe, C. Gregory, D. Croom-Carter, F. Wang, R. Longnecker, E. Kieff, and A. Rickinson, Cell 65:1107-1115, 1991). This finding is consistent a model which contributes to immortalization B cells mediated by EBV. We therefore asked whether induction three cellular phenotypes induced or associated LMP. The primary infected B95-8 strain EBV varied between 1 1.8 times that uninfected when 50% were infected, expressed LMP, incorporated 20-fold more [3H]thymidine than did cells. indicates proliferation these not sufficient induce Bcl-2. found BALB/c 3T3 their derivatives transformed LMP do express detectably. at high levels lymphoid cytotoxic following selection for introduced gene; 2 days after transfection, control vector- LMP-transfected populations, however, equal protein. also analyzed transient line. Infection BJAB results increase time course similar expression, alone transiently induces interpret observations indicate unlikely contribute ability immortalize both transformation rodent cytotoxicity are independent