Differential responses of chondrocytes from normal and osteoarthritic human articular cartilage to mechanical stimulation.
作者： George Nuki , Donald Salter , S J Millward-Sadler , M O Wright
DOI:
关键词: Paracrine signalling 、 Aggrecan 、 Autocrine signalling 、 Endocrinology 、 Stimulation 、 Cartilage 、 Chondrocyte 、 Mechanotransduction 、 Osteoarthritis 、 Internal medicine 、 Chemistry
摘要: Mechanical stimulation is critically important for the maintenance of normal articular cartilage integrity. Molecular events regulating responses chondrocytes to mechanical forces are beginning be defined. Chondrocytes from human knee joint show increased levels aggrecan mRNA following 0.33 Hz whilst at same time relative MMP3 decreased. This anabolic response, associated with membrane hyperpolarisation, activated via an integrin-dependent interleukin (IL)-4 autocrine/paracrine loop. Work in our laboratory suggests that this chondroprotective response may aberrant osteoarthritis (OA). OA no changes or stimulation. alpha5beta1 integrin mechanoreceptor both and but downstream signalling pathways differ. depolarisation consequent activation IL1beta IL4 preferentially through type I (IL4alpha/cgamma) receptor rather than II (IL4alpha/IL13R) receptor. Altered mechanotransduction contribute chondrocyte behaviour leading breakdown disease progression.
ed.ac.uk
manchester.ac.uk参考文章(33)
S. J. MillwardâSadler, M. O. Wright, L. W. Davies, G. Nuki, D. M. Salter, Mechanotransduction via integrins and interleukin-4 results in altered aggrecan and matrix metalloproteinase 3 gene expression in normal, but not osteoarthritic, human articular chondrocytes. Arthritis & Rheumatism. ,vol. 43, pp. 2091- 2099 ,(2000) , 10.1002/1529-0131(200009)43:9<2091::AID-ANR21>3.0.CO;2-C
J. P. Veldhuijzen, L. A. Bourret, G. A. Rodan, In Vitro studies of the effect of intermittent compressive forces on cartilage cell proliferation Journal of Cellular Physiology. ,vol. 98, pp. 299- 306 ,(1979) , 10.1002/JCP.1040980206
D. M. SALTER, D. E. HUGHES, R. SIMPSON, D. L. GARDNER, INTEGRIN EXPRESSION BY HUMAN ARTICULAR CHONDROCYTES Rheumatology. ,vol. 31, pp. 231- 234 ,(1992) , 10.1093/RHEUMATOLOGY/31.4.231
A.J. Grodzinsky, M.D. Buschmann, Y.A. Gluzband, E.B. Hunziker, Mechanical compression modulates matrix biosynthesis in chondrocyte/agarose culture Journal of Cell Science. ,vol. 108, pp. 1497- 1508 ,(1995) , 10.1242/JCS.108.4.1497
M. O Jortikka, R. I Inkinen, M. I Tammi, J. J Parkkinen, J. Haapala, I. Kiviranta, H. J Helminen, M. J Lammi, Immobilisation causes longlasting matrix changes both in the immobilised and contralateral joint cartilage Annals of the Rheumatic Diseases. ,vol. 56, pp. 255- 261 ,(1997) , 10.1136/ARD.56.4.255
S.J. Millward-Sadler, M.O. Wright, H.-S. Lee, K. Nishida, H. Caldwell, G. Nuki, D.M. Salter, Integrin-regulated Secretion of Interleukin 4: A Novel Pathway of Mechanotransduction in Human Articular Chondrocytes Journal of Cell Biology. ,vol. 145, pp. 183- 189 ,(1999) , 10.1083/JCB.145.1.183
Brandt Kd, Adams Me, Hypertrophic repair of canine articular cartilage in osteoarthritis after anterior cruciate ligament transection. The Journal of Rheumatology. ,vol. 18, pp. 428- 435 ,(1991)
Malcolm Wright, Paresh Jobanputra, Charles Bavington, Donald M. Salter, George Nuki, Effects of intermittent pressure-induced strain on the electrophysiology of cultured human chondrocytes: evidence for the presence of stretch-activated membrane ion channels. Clinical Science. ,vol. 90, pp. 61- 71 ,(1996) , 10.1042/CS0900061
M. O. Wright, R. A. Stockwell, G. Nuki, Response of plasma membrane to applied hydrostatic pressure in chondrocytes and fibroblasts. Connective Tissue Research. ,vol. 28, pp. 49- 70 ,(1992) , 10.3109/03008209209014227
Markku Tammi, Anna-Marja Säämänen, Anneli Jauhiainen, Outi Malminen, Ilkka Kiviranta, Heikkl Helminen, Proteoglycan Alterations in Rabbit Knee Articular Cartilage Following Physical Exercise and Immobilization Connective Tissue Research. ,vol. 11, pp. 45- 55 ,(1983) , 10.3109/03008208309015010