Human Protamine‑1 as an MRI Reporter Gene Based on Chemical Exchange
作者： Amnon Bar-Shir , Guanshu Liu , Kannie W.Y. Chan , Nikita Oskolkov , Xiaolei Song
DOI: 10.1021/CB400617Q
关键词: Protamine 、 Cloning 、 Cell biology 、 Peptide sequence 、 Biology 、 Molecular biology 、 Gene expression 、 Reporter gene 、 HEK 293 cells 、 Gene 、 Amino acid
摘要: Genetically engineered reporters have revolutionized the understanding of many biological processes. MRI-based reporter genes can dramatically improve our ability to monitor dynamic gene expression and allow coregistration subcellular genetic information with high-resolution anatomical images. We developed a biocompatible MRI based on human gene, protamine-1 (hPRM1). The arginine-rich hPRM1 (47% arginine residues) generates high contrast chemical exchange saturation transfer (CEST) mechanism. 51 amino acid- long protein was fully synthesized using microwave-assisted technology, CEST characteristics this were compared other CEST-based agents. Both bacterial cells express an optimized showed higher controls. Live expressing embedded in three-dimensional culture, also generated wild-type live cells.
acs.org
elsevier.com
sci-hub.se 参考文章(32)
Guanshu Liu, Yajie Liang, Amnon Bar-Shir, Kannie W. Y. Chan, Chulani S. Galpoththawela, Segun M. Bernard, Terence Tse, Nirbhay N. Yadav, Piotr Walczak, Michael T. McMahon, Jeff W. M. Bulte, Peter C. M. van Zijl, Assaf A. Gilad, Monitoring enzyme activity using a diamagnetic chemical exchange saturation transfer magnetic resonance imaging contrast agent. Journal of the American Chemical Society. ,vol. 133, pp. 16326- 16329 ,(2011) , 10.1021/JA204701X
Angelique Y. Louie, Martina M. Hüber, Eric T. Ahrens, Ute Rothbächer, Rex Moats, Russell E. Jacobs, Scott E. Fraser, Thomas J. Meade, In vivo visualization of gene expression using magnetic resonance imaging Nature Biotechnology. ,vol. 18, pp. 321- 325 ,(2000) , 10.1038/73780
Joy Y. Wu, Thomas J. Ribar, David E. Cummings, Kimberly A. Burton, G. Stanley McKnight, Anthony R. Means, Spermiogenesis and exchange of basic nuclear proteins are impaired in male germ cells lacking Camk4. Nature Genetics. ,vol. 25, pp. 448- 452 ,(2000) , 10.1038/78153
Karim Snoussi, Jeff W.M. Bulte, Maurice Guéron, Peter C.M. van Zijl, Sensitive CEST agents based on nucleic acid imino proton exchange: detection of poly(rU) and of a dendrimer-poly(rU) model for nucleic acid delivery and pharmacology. Magnetic Resonance in Medicine. ,vol. 49, pp. 998- 1005 ,(2003) , 10.1002/MRM.10463
Nicholas Goffeney, Jeff W. M. Bulte, Jeff Duyn, L. Henry Bryant, Peter C. M. van Zijl, Sensitive NMR detection of cationic-polymer-based gene delivery systems using saturation transfer via proton exchange. Journal of the American Chemical Society. ,vol. 123, pp. 8628- 8629 ,(2001) , 10.1021/JA0158455
John D. Lewis, Yue Song, Miriam E. de Jong, Sabira M. Bagha, Juan Ausió, A walk though vertebrate and invertebrate protamines. Chromosoma. ,vol. 111, pp. 473- 482 ,(2003) , 10.1007/S00412-002-0226-0
Guanshu Liu, Assaf A. Gilad, Jeff W. M. Bulte, Peter C. M. van Zijl, Michael T. McMahon, High-throughput screening of chemical exchange saturation transfer MR contrast agents. Contrast Media & Molecular Imaging. ,vol. 5, pp. 162- 170 ,(2010) , 10.1002/CMMI.383
Guillem Genove, Ulrike DeMarco, Hongyan Xu, William F Goins, Eric T Ahrens, A new transgene reporter for in vivo magnetic resonance imaging. Nature Medicine. ,vol. 11, pp. 450- 454 ,(2005) , 10.1038/NM1208
Jonghoon Choi, Kyobum Kim, Taeho Kim, Guanshu Liu, Amnon Bar-Shir, Taeghwan Hyeon, Michael T. McMahon, Jeff W.M. Bulte, John P. Fisher, Assaf A. Gilad, MULTIMODAL IMAGING OF SUSTAINED DRUG RELEASE FROM 3-D POLY(PROPYLENE FUMARATE) (PPF) SCAFFOLDS Journal of Controlled Release. ,vol. 156, pp. 239- 245 ,(2011) , 10.1016/J.JCONREL.2011.06.035
Anna Moore, Lee Josephson, Rajeev M. Bhorade, James P. Basilion, Ralph Weissleder, Human transferrin receptor gene as a marker gene for MR imaging. Radiology. ,vol. 221, pp. 244- 250 ,(2001) , 10.1148/RADIOL.2211001784