Monitoring enzyme activity using a diamagnetic chemical exchange saturation transfer magnetic resonance imaging contrast agent.

摘要: Chemical exchange saturation transfer (CEST) is a new approach for generating magnetic resonance imaging (MRI) contrast that allows monitoring of protein properties in vivo. In this method, radiofrequency pulse used to saturate the magnetization specific protons on target molecule, which then transferred water via chemical and detected using MRI. One advantage CEST magnetizations different can be specifically saturated at frequencies. This enables detection multiple targets simultaneously living tissue. We present here MRI detecting activity cytosine deaminase (CDase), an enzyme catalyzes deamination uracil. Our findings suggest metabolism two substrates enzyme, 5-fluorocytosine (5FC), pulses targeted +2 ppm +2.4 (with respect water), respectively. Indeed, after ...