Molecular cytogenetic characterization and SSR marker analysis of a leaf rust resistant wheat line carrying a 6G(6B) substitution from Triticum timopheevii (Zhuk.)
作者： Andrea Uhrin , Éva Szakács , László Láng , Zoltán Bedő , Márta Molnár-Láng
DOI: 10.1007/S10681-011-0483-1
关键词: Repeated sequence 、 Chromosomal translocation 、 Genetics 、 Powdery mildew 、 Biology 、 Fluorescence in situ hybridization 、 Chromosome 、 Triticum timopheevii 、 Genome 、 Microsatellite
摘要: A disease (powdery mildew, leaf rust) resistant line was selected from the progenies of a Triticum aestivum × Triticum timopheevii amphiploid produced at Martonvasar. This previously identified with C-banding as 6G(6B) substitution. In order to detect 6G chromosome in wheat background, fluorescence situ hybridization (FISH) and microsatellite marker analysis were used. Ten markers 43 tested generated PCR products that polymorphic between chromosomes 6B 6G, four showed length-polymorphism. The FISH pattern T. using combination repetitive DNA probes (Afa-family, pSc119.2, pTa71, (GAA)7). Genomic (GISH) technique, capable labelling At G genomes separately, used on same slides differentiate timopheevii. grouped basis GISH patterns cyclic intergenomic translocation involving 6At-1G-4G detected accession TRI667. presence substitution demonstrated probes. Chromosome clearly its different parental cultivar Fleischmann-481. According field tests, has resistance rust.
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sci-hub.se 参考文章(47)
Direct labelling of plant chromosomes by rapid in situ hybridization. Trends in Genetics. ,vol. 10, pp. 265- 266 ,(1994) , 10.1016/0168-9525(90)90007-S
N. S. Badaev, T. M. Enno, F. J. Zeller, H. O. Peusha, E. D. Badaeva, Chromosome substitutions in progeny of hybrids Triticum aestivum x Triticum timopheevii resistant to brown rust and powdery mildew Russian Journal of Genetics. ,vol. 31, pp. 75- 77 ,(1995)
IN Leonova, MS Röder, EB Budashkina, NP Kalinina, EA Salina, None, Molecular Analysis of Leaf Rust-Resistant Introgression Lines Obtained by Crossing of Hexaploid Wheat Triticum aestivum with Tetraploid Wheat Triticum timopheevii Russian Journal of Genetics. ,vol. 38, pp. 1648- ,(2002) , 10.1023/A:1021691822962
RA McIntosh, Y Yamazaki, J Dubcovsky, J Rogers, C Morris, R Appels, XC ed Xia, Catalogue of gene symbols for wheat International Wheat Genetics Symposium. ,vol. 4, ,(1998)
J. Lutz, E. Limpert, P. Bartos, F. J. Zeller, Identification of Powdery Mildew Resistance Genes in Common Wheat (Triticum aestivum L.). I. Czechoslovakian Cultivars Plant Breeding. ,vol. 108, pp. 33- 39 ,(1992) , 10.1111/J.1439-0523.1992.TB00097.X
Márta Molnár-Láng, Gabriella Linc, Bernd R. Friebe, József Sutka, Detection of wheat-barley translocations by genomic in situ hybridization in derivatives of hybrids multiplied in vitro Euphytica. ,vol. 112, pp. 117- 123 ,(2000) , 10.1023/A:1003840200744
Gabriella Linc, Bernd R. Friebe, Ralf G. Kynast, Marta Molnar-Lang, Bela Köszegi, Jozsef Sutka, Bikram S. Gill, Molecular cytogenetic analysis of Aegilops cylindrica host. Genome. ,vol. 42, pp. 497- 503 ,(1999) , 10.1139/GEN-42-3-497
B. Maestra, T. Naranjo, Structural chromosome differentiation between Triticum timopheevii and T. turgidum and T. aestivum Theoretical and Applied Genetics. ,vol. 98, pp. 744- 750 ,(1999) , 10.1007/S001220051130
B. Friebe, J. Jiang, W. J. Raupp, R. A. McIntosh, B. S. Gill, Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status Euphytica. ,vol. 91, pp. 59- 87 ,(1996) , 10.1007/BF00035277
L. D. Perugini, J. P. Murphy, D. Marshall, G. Brown-Guedira, Pm37, a new broadly effective powdery mildew resistance gene from Triticum timopheevii. Theoretical and Applied Genetics. ,vol. 116, pp. 417- 425 ,(2008) , 10.1007/S00122-007-0679-X