Development and characterisation of interspecific hybrid lines with genome-wide introgressions from Triticum timopheevii in a hexaploid wheat background.

摘要: Triticum timopheevii (2n = 4x = 28; AtAtGG), is an important source for new genetic variation wheat improvement with genes potential disease resistance and salt tolerance. By generating a range of interspecific hybrid lines, T. can contribute to wheat’s narrow gene-pool be practically utilised in breeding programmes. Previous studies that have generated such introgression lines between its wild relatives been unable use high-throughput methods detect the presence relative segments lines. A whole genome approach, exploiting homoeologous recombination absence Ph1 locus, has resulted transfer different chromosome from both At G genomes into wheat. These introgressions detected characterised using single nucleotide polymorphism (SNP) markers present on Axiom® Genotyping Array. The analysis these detection 276 putative unique timopheevii, thereby allowing generation map containing 1582 SNP markers, spread across 14 linkage groups representing each seven chromosomes timopheevii. genotyping was validated through fluorescence situ hybridisation (FISH). Comparative physical hexaploid showed synteny two species highly conserved at macro-level confirmed inter- intra-genomic translocations within previously only cytological techniques. In this work, we report set array, able background making it potentially valuable tool marker assisted selection (MAS) pre-breeding programs. resources high-density molecular wheat-T. will greatly enhance work being undertaken introgressions.