Biocompatibility investigation of hybrid organometallic polymers for sub-micron 3D printing via laser two-photon polymerisation

摘要: Hybrid organometallic polymers are a class of functional materials which can be used to produce structures with sub-micron features via laser two-photon polymerisation. Previous studies demonstrated the relative biocompatibility Al and Zr containing hybrid in vitro. However, deeper understanding their effects on intracellular processes is needed if tissue engineering strategy based these envisioned. Herein, primary rat myogenic cells were cultured spin-coated polymer surfaces investigate how each material affects viability, adhesion strength, adhesion-associated protein expression, rate cellular metabolism collagen secretion. We found that investigated supported growth full confluency. A subsequent MTT assay showed glass led higher rates than did surfaces. viability revealed all comparable levels cell viability. Cellular strength assessment an insignificantly stronger after 4 h culture 24 h. The largest amount was secreted by grown Al-containing surface. In conclusion, biocompatible vitro have potential for bioengineering applications.