Articular cartilage generation applying PEG-LA-DM/PEGDM copolymer hydrogels

摘要: Injuries to the human native cartilage tissue are particularly problematic because has little no ability heal or regenerate itself. Employing a engineering strategy that combines suitable cell sources and biomimetic hydrogels could be promising alternative achieve regeneration. However, weak mechanical properties may major drawback use fully degradable hydrogels. Besides, most of degrade too fast permit enough extracellular matrix (ECM) production for neocartilage formation. In this study, we demonstrated feasibility regeneration using swine articular chondrocytes photoencapsualted into poly (ethylene glycol) dimethacrylate (PEGDM) copolymer composed different degradation profiles: (PEG-LA-DM) nondegradable macromers in molar ratios 50/50, 60/40, 70/30, 80/20, 90/10. Articular were isolated enzymatically from juvenile Yorkshire cartilage. 6 × 107 cells added each milliliter macromer/photoinitiator (I2959) solution. Nonpolymerized gel containing (100 μL) was placed cylindrical molds (4.5 mm diameter 6.5 mm height). The macromer/photoinitiator/chondrocyte solutions polymerized ultraviolet (365 nm) light at 10 mW/cm2 10 mins. Also, an cartilaginous ring model used examine capacity engineered integrate with Samples pilot study collected 6 weeks. long-term experimental groups (60/40 70/30) implanted nude mice subcutaneously harvested 6, 12 18 weeks. Additionally, constructs not as time zero controls. All specimens examined grossly analyzed histologically biochemically. Histologically, formed photochemically crosslinked gels resembled lacunae surrounded by new ECM. Increases total DNA, glycosaminoglycan, hydroxyproline observed over periods studied. integrated existing generation achieved photoencapsulated PEGDM hydrogels, had adjacent