Enhancing the stiffness of collagen hydrogels for delivery of encapsulated chondrocytes to articular lesions for cartilage regeneration.

摘要: This study investigated a dual crosslinking paradigm, consisting of (a) photocrosslinking with Rose Bengal (RB) and green light followed by (b) chemical 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS) to enhance collagen gel stiffness. In group 1, 50 μL constructs 2% (w/v) type I containing 10 μM RB were allowed spontaneously at 37°C. 2, the spontaneous gels exposed (532 nm). 3, photochemically crosslinked subsequently treated 1-h exposure 33 mM EDC/6 NHS. Samples (n = 18) subjected 0.08% collagenase digestion, storage modulus samples was measured rheometry. Viability encapsulated chondrocytes live/dead assay. Chondrocytes ≥95% viable in all days vitro. Resistance digestion increased as; (2 h)  24 h). The dual-crosslinked 5-fold over both photocrosslinked gels. As paradigm did not reduce chondrocyte viability, these hydrogels could be useful tool for practical delivery articular defects. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1332–1338, 2015.