Isoprenylation of rap2 proteins in platelets and human erythroleukemia cells.
作者： D A Winegar , L Molina y Vedia , E G Lapetina
DOI: 10.1016/S0021-9258(20)64333-2
关键词: Recombinant DNA 、 Immunoprecipitation 、 G protein 、 Platelet 、 Prenylation 、 Biology 、 Antiserum 、 Polyclonal antibodies 、 Mevalonic acid 、 Biochemistry
摘要: The covalent modification of proteins by isoprenoid derivatives mevalonic acid was investigated in human platelets, cells that lack the ability to synthesize endogenous cholesterol, and erythroleukemia (HEL) cells, cholesterol-producing cultured derived from megakaryocytes. When washed platelets or HEL were incubated with [3H]mevalonic acid, radiolabel incorporated into a distinct group molecular masses between 21,000 28,000. We have identified one these as ras-related rap2 protein based on its immunoreactivity polyclonal antiserum raised against purified recombinant rap2b. This anti-rap2 used for two-dimensional immunoblotting analysis immunoprecipitation mevalonate-labeled cells. These results suggest may undergo series carboxyl-terminal modifications similar p21ras proteins. In addition, it is shown non-cholesterol-producing are capable incorporating isoprenyl groups specific
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