A competitive direct enzyme-linked immunosorbent assay for the rapid detection of deoxynivalenol: development and application in agricultural products and feedstuff
作者： Yan Zhang , Jian Yang , Yang Lu , Dong-Yue Ma , Mu Ge Qi
DOI: 10.1080/09540105.2017.1306491
关键词: Enzyme 、 Detection limit 、 Chromatography 、 Rapid detection 、 High-performance liquid chromatography 、 Extraction (chemistry) 、 Chemistry 、 Dilution 、 Polyclonal antibodies
摘要: ABSTRACTA competitive direct enzyme-linked immunosorbent assay (cd-ELISA) was developed for the rapid detection of deoxynivalenol (DON) in food and feedstuff. Polyclonal antibodies against DON were generated by immunizing rabbits with 3-HS-DON-BSA conjugates. In this assay, sensitivity (measured as IC50) limit IC15) 0.03 0.003 mg kg−1, respectively. A total eight sample types (barley, wheat, oat, maize, rice, flour, milk feedstuff) chosen to evaluate cd-ELISA performance. The could be directly detected after extraction dilution double-distilled water. samples range 0.15–0.48 mg kg−1. end, spike recovery results both high-performance liquid chromatography (HPLC) compared validation. ranged between 70% 100%. good correlation (R2 = 0.9613) ELISA HPLC obtained.
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