Magnetic nanoparticles replacing microplate as immobile phase could greatly improve the sensitivity of chemiluminescence enzymatic immunoassay for deoxynivalenol
作者： Songcheng Yu , Fei Yu , Yaping Li , Lie Liu , Hongquan Zhang
DOI: 10.1016/J.FOODCONT.2015.08.012
关键词: Phase (matter) 、 Chemiluminescence 、 Chemistry 、 Immunoassay 、 Detection limit 、 Chromatography 、 Linear range 、 Magnetic nanoparticles 、 Microparticle 、 Magnetic separation
摘要: Abstract Deoxynivalenol, a secondary metabolite of Fusarium graminearum, is suspected carcinogen. It has great threat to people's health. A sensitive determination method for deoxynivalenol necessary satisfy food safety requirement. In this study, traditional chemiluminescent enzyme immunoassay (CLEIA) was modified by replacing the microplate with magnetic nanoparticle as immobile phase. The limit detection novel CLEIA low 0.032 ng/mL. linear range 0.1–20 ng/mL. within-run and between-run precisions were 2.1%–4.7% 5.4%–10.0%, respectively. And showed good specificity. Therefore, it could be concluded that nanoparticles phase improve sensitivity precision through merits microparticle separation. This significant work safety.
elsevier.com
sci-hub.se 参考文章(22)
Kaori Morota, Ryo Fujinami, Hideki Kinukawa, Taiji Machida, Kenichi Ohno, Haruhisa Saegusa, Katsumichi Takeda, A new sensitive and automated chemiluminescent microparticle immunoassay for quantitative determination of hepatitis C virus core antigen. Journal of Virological Methods. ,vol. 157, pp. 8- 14 ,(2009) , 10.1016/J.JVIROMET.2008.12.009
Z.H. Shar, G.A. Sumbal, S.T.H. Sherazi, M.I. Bhanger, S.M. Nizamani, Natural co-occurrence of aflatoxins and deoxynivalenol in poultry feed in Pakistan. Food Additives & Contaminants Part B-surveillance. ,vol. 7, pp. 162- 167 ,(2014) , 10.1080/19393210.2013.867904
Eliana Castañares, Diana Ramirez Albuquerque, María Inés Dinolfo, Virginia Fernandez Pinto, Andrea Patriarca, Sebastián Alberto Stenglein, Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina. International Journal of Food Microbiology. ,vol. 179, pp. 57- 63 ,(2014) , 10.1016/J.IJFOODMICRO.2014.03.024
Arnaud Hallier, Florian Celette, Julie Coutarel, Christophe David, A contribution to reduce sampling variability in the evaluation of deoxynivalenol contamination of organic wheat grain Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment. ,vol. 30, pp. 2159- 2164 ,(2013) , 10.1080/19440049.2013.853227
Maria Magliulo, Mara Mirasoli, Patrizia Simoni, Rosella Lelli, Ottavio Portanti, Aldo Roda, None, Development and validation of an ultrasensitive chemiluminescent enzyme immunoassay for aflatoxin M1 in milk. Journal of Agricultural and Food Chemistry. ,vol. 53, pp. 3300- 3305 ,(2005) , 10.1021/JF0479315
Marijana Sokolović, Borka Šimpraga, Survey of trichothecene mycotoxins in grains and animal feed in Croatia by thin layer chromatography Food Control. ,vol. 17, pp. 733- 740 ,(2006) , 10.1016/J.FOODCONT.2005.05.001
Yuanbi ZHAO, Zumin QIU, Jiaying HUANG, Preparation and Analysis of Fe3O4 Magnetic Nanoparticles Used as Targeted-drug Carriers * Chinese Journal of Chemical Engineering. ,vol. 16, pp. 451- 455 ,(2008) , 10.1016/S1004-9541(08)60104-4
James J. Pestka, Deoxynivalenol: mechanisms of action, human exposure, and toxicological relevance. Archives of Toxicology. ,vol. 84, pp. 663- 679 ,(2010) , 10.1007/S00204-010-0579-8
Ikuo Sato, Michihiro Ito, Masumi Ishizaka, Yoko Ikunaga, Yukari Sato, Shigenobu Yoshida, Motoo Koitabashi, Seiya Tsushima, Thirteen novel deoxynivalenol-degrading bacteria are classified within two genera with distinct degradation mechanisms Fems Microbiology Letters. ,vol. 327, pp. 110- 117 ,(2012) , 10.1111/J.1574-6968.2011.02461.X
Felicia Wu, John D. Groopman, James J. Pestka, Public Health Impacts of Foodborne Mycotoxins Annual Review of Food Science and Technology. ,vol. 5, pp. 351- 372 ,(2014) , 10.1146/ANNUREV-FOOD-030713-092431