Development and validation of an ultrasensitive chemiluminescent enzyme immunoassay for aflatoxin M1 in milk.

摘要: A fast and ultrasensitive chemiluminescent enzyme immunoassay for aflatoxin M1 in milk samples has been developed validated. The method is an indirect competitive type format involving the immobilization of M1−bovine serum albumin conjugate on 384 well black polystyrene microtiter plates use a secondary antibody labeled with horseradish peroxidase detected luminol-based substrate. Aflatoxin standard solutions were prepared milk-based buffer, analyzed without any cleanup procedure. limit quantification was 1 ppt, coefficient variation below 9% both intra- interassay precision, recovery ranged from 96 to 122%. specific, other aflatoxins do not significantly cross-react antibody. Twenty-four analyzed, good correlation observed (y = 0.98x + 1.71, r2 0.98, n 24) when data compared reference high-performance liquid chromatography ...