A new sensitive and automated chemiluminescent microparticle immunoassay for quantitative determination of hepatitis C virus core antigen.
作者： Kaori Morota , Ryo Fujinami , Hideki Kinukawa , Taiji Machida , Kenichi Ohno
DOI: 10.1016/J.JVIROMET.2008.12.009
关键词:
摘要: Abstract A new sensitive and automated chemiluminescent assay was developed for the quantitative determination of hepatitis C virus (HCV) core antigen (Ag) in human sera or plasma: Abbott ARCHITECT ® HCV Ag test. The sensitivity determined by testing 10 commercial seroconversion panels. Without exception, a positive result observed before anti-HCV detection, resulting an average reduction period between exposure detection 35.8 days. Both RNA were detected panels at same time, indicating equivalent detectability. total 197 specimens comprising genotypes 1a, 1b, 2a, 2b, 3a, 3k, 4a, 5a 6a evaluated. Among these, 196 (99.5%), 191 (97%) 193 (98%) reactive using Ag, immunoradiometric Amplicor Monitor 2 assays, respectively. comparison with showed correlation coefficient ( r ) 0.74. specificity established 99.8% 5403 from US volunteer blood donors, hospitalized patients individuals medical conditions unrelated to infection, addition containing potentially interfering substances.
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