Autotaxin is overexpressed in glioblastoma multiforme and contributes to cell motility of glioblastoma by converting lysophosphatidylcholine to lysophosphatidic acid.

摘要: Autotaxin (ATX) is a multifunctional phosphodiesterase originally isolated from melanoma cells as potent cell motility-stimulating factor. ATX identical to lysophospholipase D, which produces bioactive phospholipid, lysophosphatidic acid (LPA), lysophosphatidylcholine (LPC). Although enhanced expression of in various tumor tissues has been repeatedly demonstrated, and thus, implicated progression tumor, the precise role expressed by was unclear. In this study, we found that highly glioblastoma multiforme (GBM), most malignant glioma due its high infiltration into normal brain parenchyma, but not other tumors. addition, LPA1, an LPA receptor responsible for LPA-driven motility, predominantly GBM. One glioblastomas showed highest (SNB-78), well ATX-stable transfectants, LPA1-dependent migration response both Boyden chamber wound healing assays. Interestingly these ATX-expressing also chemotactic LPC. knockdown level using small interfering RNA technique SNB-78 suppressed their migratory These results suggest autocrine production cancer cell-derived exogenously supplied LPC contribute invasiveness ATX, LPC-producing enzymes are potential targets therapy, including