A collaborative study of a method for enumeration of probiotic enterococci in animal feed
作者： R.G.K. Leuschner , J. Bew , K.J. Domig , W. Kneifel
DOI: 10.1046/J.1365-2672.2002.01755.X
关键词: Food science 、 Enumeration 、 Agar 、 Relative standard deviation 、 Routine analysis 、 Single component 、 Probiotic 、 Biology 、 Animal feed 、 Control methods
摘要: Aims: Validation of an enumeration method to be used as official control in the framework Council Directive 70/524/EEC for probiotic enterococci feed additives. Methods and Results: Twenty laboratories 12 European countries carried out a collaborative study. A plate count using bile esculin azide (BEA) agar was used. Precision data terms repeatability (r) reproducibility (R) different feeding stuffs three inoculation levels were determined. Enterococci present samples single component or mixtures with other additives. The on BEA showed relative standard deviation (RSD)r 1·5–3·6% RSDR between 2·9 7·4%. selective presence micro-organisms such pediococci, lactobacilli yeast. Conclusions: For routine analysis viable concentrations stuffs, use is recommended. This methodology not applicable mineral feeds. Significance Impact Study: An authorized validated. results are intended consideration adoption CEN ISO standards.
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