Culture media for enterococci and group D-streptococci

摘要: Lancefield group D-streptococci are contaminants of various food commodities, especially those animal origin. They encompass the new genus Enterococcus comprising 13 known species and some streptococci which have their habitat in intestine animals, e.g. Streptococcus bovis, suis equinus. The serologically based grouping may no longer constitute best definition for from chain. Food hygiene monitoring systems using enterococci as indicators need reliable methods selective cultivation identification marker strains. Up to now more than 100 modifications media been described isolating or specimens. selection a medium requires either experience consultation. It depends on kind specimen, method (plate count membrane filter) whether not is heavily contaminated with other organisms. choice made difficult commercial versions same culture vary recipe and/or performance producer producer. Therefore, reviewing literature help confirmation tests. selectivity productivity commonly used cited reported here, partly our own experience: citrate azide tween carbonate agar (CATC), kanamycin aesculin (KAA) M-enterococcus (ME) including earlier results bile (ABA), thallous acetate tetrazolium glucose (TITG). No was completely all but were highly single species, e.g., E. faecalis serves indicator human pollution. Confirmatory tests must be carried out when evaluation procedure limited. Selective should only after while checking parallel against appropriate test