作者: Mireille Serhan , Catherine Cailliez-Grimal , Frédéric Borges , Anne-Marie Revol-Junelles , Chadi Hosri
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摘要: Abstract In order to contribute the preservation of Lebanese dairy heritage, aim this study was characterize Darfiyeh cheese, a traditional variety made from raw goat's milk and ripened in skin. Three independent batches production were analyzed after 20, 40 60 days ripening. Mesophilic lactobacilli, thermophilic coccal-shaped lactic acid bacteria (LAB) lactobacilli enumerated. explore natural ecosystem, combination phenotypical molecular approaches applied. The latter included Polymerase Chain Reaction-temporal temperature gel electrophoresis (PCR-TTGE), classical PCR quantitative PCR. These methods revealed presence Streptococcus thermophilus , Enterococcus faecium durans faecalis malodoratus group D sp., Lactococcus lactis subsp. L. cremoris Lactobacillus plantarum curvatus Staphylococcus haemolyticus Escherichia coli Clostridium sp./ Eubacterium tenue . Real-time enabled quantification E. with detection 10 7 –10 9 cfu g −1 product. present combined phenotypic method allowed describing complex ecosystem Darfiyeh, giving useful information for artisanal products.