Antibiotic Resistance Patterns of Enterococci and Occurrence of Vancomycin-Resistant Enterococci in Raw Minced Beef and Pork in Germany

作者: G�nter Klein , Alexander Pack , Gerhard Reuter

DOI: 10.1128/AEM.64.5.1825-1830.1998

关键词: Food scienceEnterococcusAgar plateTeicoplaninAgarAvoparcinBiologyBroth microdilutionVancomycinMinced beefMicrobiology

摘要: The food chain, especially raw minced meat, is thought to be responsible for an increase in the incidence of vancomycin-resistant enterococci (VRE) human nosocomial infections. Therefore, 555 samples from 115 batches beef and pork a European Union-licensed meat-processing plant were screened occurrence VRE. processed meat came 45 different slaughterhouses Germany. Enterococci isolated directly Enterococcosel selective agar plates also supplemented with 32 mg vancomycin per liter. In addition, peptone broth was used preenrichment procedure, subsequently plated onto containing vancomycin. To determine resistance, 209 isolates 275 tested glycopeptides vancomycin, teicoplanin, avoparcin 19 other antimicrobial substances by using microdilution test. When direct method used, VRE found 3 (0.5%) at concentration 1.0 log CFU/g meat. procedure 8% positive. Our findings indicate that there low resistance patterns obtained clinical isolates. A connection between infections could not demonstrated on basis our findings.

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