Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions

摘要: Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits largely recognized. Nevertheless, major physicochemical limitations, such as high instability of catechins, pose important questions concerning their potential pharmacological use. Recent studies indicate that binding catechins with plasmatic proteins may modulate plasma concentration, tissue delivery biological activity. After 5 minutes incubation HSA both ECG EGCG were fully bound to HSA, while after 48h only 41% EC 70% EGC resulted linked. had strong stabilizing effect on all which could be found solution between 29 85% even incubation. In absence disappeared less than 24h, at 50%, respectively. The toward EGCG, obtained aqueous physiological conditions, stronger comparison cysteine HCl, previously reported stabilize this polyphenol. Because multitude contradictory data vivo vitro antioxidant-based experimentations, we believe our work shed some light debated field research.