Accuracy of oxygen measurements in T2 -(line width) EPR oximetry
作者： Tatyana I. Smirnova , Alex I. Smirnov , Robert B. Clarkson , R. Linn Belford
关键词: Chemistry 、 Line (formation) 、 Oxygen 、 Homogeneous broadening 、 Spectral line 、 Analytical chemistry 、 Electron paramagnetic resonance 、 Proton 、 Limiting oxygen concentration 、 Nitroxide mediated radical polymerization
摘要: EPR oximetry is used for in vivo and vitro measurements of oxygen biological systems, including experimental animals. The accuracy T2 (line width) significantly improved if least-squares simulation to extract the line width parameters. effect on spectra nitroxide solutions aqueous suspensions fusinite can be described as an additional homogeneous broadening that modifies spectrum oxygen-free probe. This allows one use a one-parameter model most cases. simulations were carried out with fast-convolution algorithm followed by Levenberg-Marquardt optimization. validity error estimates provided this method was tested sets taken under common conditions. It shown (T2) determined not only probe sensitivity (rate change versus concentration), but also signal-to-noise ratio, inhomogeneous contribution shape (e.g., unresolved proton superhyperfine structure), spectral window. compared two radicals different structure fusinite.
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