作者: Patricia D. English , Joseph Byrne Jurale , Peter Albersheim
DOI: 10.1104/PP.47.1.1
关键词: Cell wall 、 Microbiology 、 Secretion 、 Enzyme 、 Enzyme assay 、 Pectinase 、 Cellulase 、 Polysaccharide 、 Biochemistry 、 Biology 、 Colletotrichum lindemuthianum 、 Plant science 、 Genetics 、 Physiology
摘要: The effect of a number physiological variables on the secretion polysaccharide-degrading enzymes by culture-grown Colletotrichum lindemuthianum (Saccardo and Magnus) Scribner was determined. spores used to inoculate cultures grown isolated bean hypocotyl cell walls affects time after inoculation at which enzyme occurs, but has no significant maximal amount ultimately secreted. Cell from leaves, first internodes, or hypocotyls (susceptible C. infection), when as carbon source for growth, stimulate fungus secrete more alpha-galactosidase than do roots (resistant infection). concentration fungal growth determines final level activity in culture fluid. is not proportional growth; rather, induced. Maximal medium found 1% greater. A higher necessary alpha-arabinosidase activity. Galactose, source, stimulates but, comparable concentrations, less effective doing so are walls. Polysaccharide-degrading secreted different times during pathogen Pectinase first, followed beta-xylosidase cellulase, then beta-glucosidase, and, finally, alpha-galactosidase.