Host-Pathogen Interactions

作者: Patricia D. English , Joseph Byrne Jurale , Peter Albersheim

DOI: 10.1104/PP.47.1.1

关键词: Cell wallMicrobiologySecretionEnzymeEnzyme assayPectinaseCellulasePolysaccharideBiochemistryBiologyColletotrichum lindemuthianumPlant scienceGeneticsPhysiology

摘要: The effect of a number physiological variables on the secretion polysaccharide-degrading enzymes by culture-grown Colletotrichum lindemuthianum (Saccardo and Magnus) Scribner was determined. spores used to inoculate cultures grown isolated bean hypocotyl cell walls affects time after inoculation at which enzyme occurs, but has no significant maximal amount ultimately secreted. Cell from leaves, first internodes, or hypocotyls (susceptible C. infection), when as carbon source for growth, stimulate fungus secrete more alpha-galactosidase than do roots (resistant infection). concentration fungal growth determines final level activity in culture fluid. is not proportional growth; rather, induced. Maximal medium found 1% greater. A higher necessary alpha-arabinosidase activity. Galactose, source, stimulates but, comparable concentrations, less effective doing so are walls. Polysaccharide-degrading secreted different times during pathogen Pectinase first, followed beta-xylosidase cellulase, then beta-glucosidase, and, finally, alpha-galactosidase.

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