Determination of methionine in protein hydrolysates by reaction with raney nickel and infrared spectroscopy

摘要: An infrared spectrophotometric determination of methionine in protein hydrolysates is suggested. The method based on the desulphurizing action specially purified Raney nickel methionine, during which methane quantitatively liberated, then measured at 3020.3 cm-1 Cystine and cysteine do not interfere can usually be determined from difference between total sulphur sulphur. applied to hydrolysates, homogenates internal organs. time required for a single about 40 min; relative error 1.2% down level 200 μg.