Metabolites of arsenic and increased DNA damage of p53 gene in arsenic plant workers

摘要: Recent studies have shown that monomethylarsonous acid is more cytotoxic and genotoxic than arsenate arsenite, which may attribute to the increased levels of reactive oxygen species. In this study, we used hydride generation-atomic absorption spectrometry determine three arsenic species in urine workers who had been working plants,and calculated primary secondary methylation indexes. The damages exon 5, 6, 8 p53 gene were determined by method developed Sikorsky, et al. Results show concentrations each urinary species,and damage indexes 5 exposed population significantly higher, but SMI was lower control group. closely positive correlation between index PMI,MMA, DMA found, there negative SMI. Those findings suggested DNA existed occupationally arsenic. For important factors include model metabolic transformation, MMA DMA, be great importance.