Microsatellite loci to recognize species for the cheese starter and contaminating strains associated with cheese manufacturing

摘要: Abstract We report the development of 17 microsatellite markers in cheese fungi Penicillium camemberti and P. roqueforti, using an enrichment protocol. Polymorphism cross-amplification were explored 23 isolates camemberti, 26 2 each chrysogenum nalgiovense species, used to produce meat fermented products. The appeared useful for differentiating both their amplification sizes sequences flanking regions. locus PC4 was particularly suitable distinguishing contaminant species closely related clarifying phylogenetic relationship this with its supposed ancestral form, commune. analyzed 22 from different culture collections assigned morphospecies commune, most them occurring as food spoilers, mainly environment. None exhibited identical ex-type isolate They instead distributed into two other distinct lineages, corresponding old fuscoglaucum biforme, previously synonymised respectively commune camemberti. strictly at all loci examined. caseifulvum, a non toxinogenic described new candidate fermentation, also may therefore be considered barcode these economically important species.