Phosphorylations of the Subcellular Matrix in Cells Transformed by ROUS' Sarcoma Virus
作者： Gerard GACON , Jean-Pierre PIAU , Sylvie GISSELBRECHT , Mare-Yves FISZMAN , Sigmund FISCHER
DOI: 10.1111/J.1432-1033.1982.TB06704.X
关键词: Phosphorylation 、 Tyrosine 、 Cytoskeleton 、 Mutant 、 Rous sarcoma virus 、 Protein kinase A 、 Phosphoprotein 、 Cell 、 Molecular biology 、 Biology
摘要: The transforming protein of Rous' sarcoma virus (RSV) is a phosphoprotein Mr 60000 (pp60src) which displays kinase activity specific for tyrosine residues; pp60src associated with the plasma membrane and recovered in detergent-insoluble material represents subcellular matrix cell. After phosphorylation this RSV-transformed cells [γ-32P]ATP, five phosphoproteins have been detected are not seen normal cells. These proteins (Mr= 135000, 125000, 75000, 70000, 60000) contain phosphotyrosine. Their strongly inhibited by anti-pp60src antibodies. In transformed temperature-sensitive mutant RSV, these phosphoproteins, present at permissive temperature, no longer non-permissive temperature. It concluded that phosphorylations mediated activity. This supports possible role cytoskeletal transformation process.
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