Localization of the Abelson murine leukemia virus protein in a detergent-insoluble subcellular matrix: architecture of the protein.

摘要: We examined the interaction of Abelson murine leukemia virus protein P120 with other cellular components after extraction nonionic detergent Triton X-100. Most P120-associated kinase activity was found in detergent-insoluble matrix both lymphoid and fibroblast cell lines. The labeled during a short exposure cells to [35S]-methionine mainly (lymphoid cells) or equally distributed soluble fraction (fibroblasts). Steady-state-labeled two fractions mostly portion Thus, there an apparent movement from detergent-soluble concomitant loss enzymatic activity. When incubated [32P]ATP situ, phosphorylation tyrosine residues observed. 80,000-molecular-weight fragment (designated F80) detergent. F80 not extracted cells, but mixing unlabeled fibroblasts before produced fragment. contained gag determinants did react Abelson-specific serum. These data allowed us assign various features regions molecule localize antigenic C-terminal region molecule.