Transplantation of Corneal Stroma Reconstructed with Gelatin and Multipotent Precursor Cells from Corneal Stroma

摘要: The cornea is composed of a multilayered epithelium, Bowman's membrane, stroma, Descemet's membrane and endothelium. As compared to the tissue engineering corneal epithelium or endothelium relatively simple well established, as stroma mainly continuous cells joined by tight junctions with few subcellular extracellular matrix (ECM) proteins. Corneal stromal engineering, however, has yet be fully achieved due difficulties encountered when trying constitute an artificial which consists fibroblast-like keratocytes, nerve fibers, transparent ECM collagen fibrils proteoglycans. Stem progenitor are defined their capacity for self-renewal ability generate different types (multipotentiality), leads formation mature tissues. In contrast, precursor unipotent limited proliferative activity. Regenerative stem precursors can detected sphere-forming assay in various adult tissues, including central nervous system (Nunes et al., 2003), bone marrow (Krause 2001), skin (Kawase 2004; Toma retina (Coles 2004), (Mimura 2010a), (Amano 2006; Mimura 2008a, 2008b; Uchida 2005; Yamagami 2007), 2005a, 2005b, 2005c, 2007, 2010b; 2006a, 2007; Yokoo 2005). Despite many successes isolation characterization from studies have investigated efficacy cell transplantation therapy animals. While three-dimensional structure that responsible maintaining cell-to-cell interactions indispensable using cells, structural complexity involved does not easily allow us extend investigations transplantations. this chapter, we introduce our recent work about regenerative medicine multipotent cells. We isolated propensity develop into keratocyte-like rabbit corneas distribution derived peripheral regions assay.