Sensitive Detection of Viruses in Pollen Using Conventional and Real-time Reverse Transcription-polymerase Chain Reaction
作者： Jason B. Shiller , Bénédicte S. M. Lebas , Mary Horner , Michael N. Pearson , Gerard R. G. Clover
DOI: 10.1111/J.1439-0434.2010.01693.X
关键词: Biology 、 Reverse transcription polymerase chain reaction 、 Pollen 、 Virology 、 Pollination 、 Bromoviridae 、 Nepovirus 、 Ilarvirus 、 Plant virus 、 Tobacco ringspot virus 、 Agronomy and Crop Science 、 Plant science 、 Genetics 、 Physiology
摘要: Pollen is traded internationally as a source of germplasm and for pollination. Thirty-nine viruses five viroids are known to be pollen transmitted. We investigated whether reverse transcription-polymerase chain reaction (RT-PCR) could used detect reliably in pollen. Four extraction methods yielded nucleic acid appropriate quantity quality from Tobacco ringspot virus (TRSV)-infected RT-PCR
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