Genetic diversity among elite Bulgarian barley varieties evaluated by RFLP and RAPD markers
作者： Elena Todorovska , Adelina Trifonova , Atanas Atanassov
关键词: Genetic diversity 、 Genetics 、 Genetic marker 、 Ribosomal DNA 、 Genetic variability 、 Genetic variation 、 Hordeum vulgare 、 Biology 、 RAPD 、 Restriction fragment length polymorphism
摘要: Genetic variation among five elite winter barley cultivars (H. vulgare L.) currently grown in Bulgaria was assessed at the molecular level using restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD) markers. The present study sampled RFLPs four well characterized multigene families barley: seed storage protein loci; 18S, 5.8S 26S ribosomal loci coding for 5S RNA subunit α of ATP-A complex mitochondrial genome. were detected three out investigated chromosomal studied. RAPD assay arbitrary 10-base primers applied to generate markers barley. Overall a total 15 phenotypes found studied by 11 25 tested primers. All RAPDs considered as dominant genetic except two, where PCR Southern blot analysis indicated presence codominant amplification products. Five polymorphisms F1 F2 progenies cross between Alpha Obzor inherited Mendelian fashion. determined values proved high similarity cultivars. (GS) calculated from RFLP data ranged 0.888 0.997 with mean GS – 0.933.
springer.com
sci-hub.se 参考文章(39)
Andris Kleinhofs, Andrzej Kilian, RFLP maps of barley Advances in Cellular and Molecular Biology of Plants. ,vol. 1, pp. 163- 198 ,(1994) , 10.1007/978-94-011-1104-1_10
Linda Bonen, Poppo H. Boer, Michael W. Gray, The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize The EMBO Journal. ,vol. 3, pp. 2531- 2536 ,(1984) , 10.1002/J.1460-2075.1984.TB02168.X
M. A. Saghai Maroof, R. A. Jorgensen, R. W. Allard, Qifa Zhang, Genetic and molecular organization of ribosomal DNA (rDNA) variants in wild and cultivated barley. Genetics. ,vol. 126, pp. 743- 751 ,(1990) , 10.1093/GENETICS/126.3.743
R. Appels, W. L. Gerlach, E. S. Dennis, H. Swift, W. J. Peacock, Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNAs in cereals. Chromosoma. ,vol. 78, pp. 293- 311 ,(1980) , 10.1007/BF00327389
A. Kleinhofs, A. Kilian, M. A. Saghai Maroof, R. M. Biyashev, P. Hayes, F. Q. Chen, N. Lapitan, A. Fenwick, T. K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, D. Kudrna, J. Bollinger, S. J. Knapp, B. Liu, M. Sorrells, M. Heun, J. D. Franckowiak, D. Hoffman, R. Skadsen, B. J. Steffenson, A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theoretical and Applied Genetics. ,vol. 86, pp. 705- 712 ,(1993) , 10.1007/BF00222660
M. A. Saghai-Maroof, K. M. Soliman, R. A. Jorgensen, R. W. Allard, Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 8014- 8018 ,(1984) , 10.1073/PNAS.81.24.8014
Masatoshi Nei, Wen-Hsiung Li, Mathematical model for studying genetic variation in terms of restriction endonucleases Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 5269- 5273 ,(1979) , 10.1073/PNAS.76.10.5269
Juan Manuel González, Esther Ferrer, Random amplified polymorphic DNA analysis in Hordeum species. Genome. ,vol. 36, pp. 1029- 1031 ,(1993) , 10.1139/G93-137
Y. Loarce, R. Gallego, E. Ferrer, A comparative analysis of the genetic relationships between rye cultivars using RFLP and RAPD markers Euphytica. ,vol. 88, pp. 107- 115 ,(1996) , 10.1007/BF00032441
M. Heun, A. E. Kennedy, J. A. Anderson, N. L. V. Lapitan, M. E. Sorrells, S. D. Tanksley, Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome. ,vol. 34, pp. 437- 447 ,(1991) , 10.1139/G91-066