Correlation of donor age and telomerase activity with in vitro cell growth and replicative potential for dermal fibroblasts and keratinocytes

摘要: Previous studies suggested telomerase activity as a determinant of cell replicative capacity by delaying senescence. This study aimed to evaluate the feasibility adopting selection criterion for in vitro expanded skin cells before autologous transplantation. Fibroblasts and keratinoctyes were derived from same consenting patients aged 9-69 years, cultured separately serum-supplemented serum-free media, respectively. Telomerase fresh measured correlated with growth rate, donor age passage number. The results showed that independent both types. was expressed freshly digested epidermis dermis continued expressing vitro. Keratinocytes consistently 3-12 folds greater than fibroblast vivo Conversely, rate exceeded keratinocyte. decreased markedly at Passage 6 keratinocytes ceased 3 fibroblasts. decrease or cessation coincided senescence keratinocyte but not fibroblast, implying telomerase-regulated former hence predictor this type. Relative fibroblasts younger group significantly higher older group; 69.7% isolates 31.1% P0 (p<0.05). No detectable be found later subcultures groups. Similarly keratinocytes, (p<0.05) compared 507.7% P0, 36.8% P3 difference no longer significant P6. In conclusion, provided evidence sustained proliferation is an important survival replication its positive detection transplantation desirable. Inferring our results, use lesser construction substitute cell-based therapy recommended owing their expression.