Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins

摘要: Advances in the life sciences are now closely linked to availability of new experimental tools that allow for manipulation biomolecules and study biological processes at molecular level. In this context, we have optimized a synthesis process obtain glutathione-capped fluorescent CdS nanoparticles specifically detect Glutathione S-Transferase (GST) -tagged proteins. Using our method, based on five different heating steps, brightly biocompatible quantum dots sizes can be obtained. QD optical behaviour has been evaluated studying both absorbance fluorescence. For all samples, excitonic absorption onset clearly shows blue shift 512nm comparison with bulk CdS, due confinement effect. At increased average nanocrystal, emission band red shift, from 440nm 540nm. Among solutions, demonstrate an expansion range up �˝ 100 nm, thus improving their features as biomarkers. Moreover show glutathione- capped directly bind GST blotted onto polyvinylidene difluoride (PVDF) membranes, suitable direct detection fusion