Quantification of ratios of Bacteria and Archaea in methanogenic microbial community by fluorescence in situ hybridization and fluorescence spectrometry
作者： Tiong-Lee Stephen Tay , Volodymyr Ivanov , In Soo Kim , Lin Feng , Joo-Hwa Tay
关键词: Bacteria 、 Archaea 、 16S ribosomal RNA 、 Biology 、 Molecular probe 、 Chromatography 、 Oligonucleotide 、 Molecular biology 、 Fluorescence in situ hybridization 、 Fluorescence spectrometry 、 Anaerobic digestion
摘要: 16S rRNA-targeted oligonucleotide probes for Bacteria (Eub338) and Archaea (Arc915) were used whole-cell, fluorescence in situ hybridization (FISH) to quantify the ratio of these microbial groups an anaerobic digester. The quantity specifically bound (hybridized) probe was measured by spectrometry evaluated analysing dissociation curve hybrids, measurement binding with a nonsense probe, competitive inhibition labelled corresponding unlabelled probe. Specific biomass anaerobes 40–50% their total binding. Arc915 Eub338 hybridized rRNAs cells sludge 0.50. Measurement FISH appears be suitable method quantification community anaerobes.
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