Characterization of Borrelia lusitaniae sp. nov. by 16S Ribosomal DNA Sequence Analysis
作者： A. L. Fleche , D. Postic , K. Girardet , O. Peter , G. Baranton
DOI: 10.1099/00207713-47-4-921
关键词: Complete sequence 、 Phylogenetic tree 、 Borrelia lusitaniae 、 Borrelia burgdorferi 、 Ribosomal DNA 、 Borrelia 、 Biology 、 Borrelia valaisiana 、 Genetics 、 Sequence analysis
摘要: We determined the complete sequence of rrs gene from five strains genomic species PotiB2. Both distance and parsimony methods were used to infer evolutionary relationships this in comparison with Borrelia valaisiana sequences burgdorferi sensu lato obtained databases. The phylogenetic analysis revealed that PotiB2 clustered a separate lineage, which was consistent data previous DNA-DNA hybridization experiments (D. Postic, M. V. Assous, P. A. D. Grimont, G. Baranton, Int. J. Syst. Bacteriol. 44:743–752, 1994). A PCR-restriction fragment length polymorphism identify differentiate it B. species. Moreover, signature nucleotide positions identified for each In accordance DNA relatedness values, our findings suggest can be more clearly defined identified, we propose should referred as new species, lusitaniae. type strain is
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