Immune precipitation and immunoblotting for the detection of Trypanosoma cruzi antigens.

摘要: Abstract The efficiency of immunoprecipitation and immunoblotting for detecting Trypanosoma cruzi antigens using rabbit mouse antisera has been examined. Comparison the starting material each technique showed that extraction methods resulted in a similar range polypeptides when assessed by apparent Mr. Reaction with either hyperimmune sera or sequential series from infected mice, significant disparity revealed technique. Epitopes on greater than 50kDa were poorly preserved compared to immunoprecipitation; however, below this threshold both techniques equally efficient. Under conditions assay, it is probable allows effective detection sequence conformational determinants, whereas favours determinants alone.