An improved strategy and a useful housekeeping gene for RNA analysis from formalin-fixed, paraffin-embedded tissues by PCR.

摘要: Specific amplification of nucleic acid sequences by PCR has been extensively used for the detection gene rearrangements and expression. Although successful DNA carried out with prepared from formalin-fixed, paraffin-embedded (FFPE) tissues, there are only a few reports regarding RNA analysis in this kind material. We describe procedure extraction different types FFPE involving digestion proteinase K followed guanidinium-thiocyanate phenol DNase I digestion. These preparations suitable mRNA even intronless genes. Furthermore, universally expressed porphobilinogen deaminase proved to be useful as positive control because lack pseudogenes.