Application of Beclin1 protein acetylation and mutation
作者： Yang Ying , Lyu Pengfei , Yang Jian , Zhang Zhiguo , Ning Guang
DOI:
关键词: Transfection 、 Cell biology 、 Mutant 、 Point mutation 、 Autophagy 、 Histone deacetylase inhibitor 、 Acetylation 、 Mutation 、 Plasmid 、 Chemistry
摘要: The invention relates to the fields of physiology and molecular biology discloses an application Beclin1 acetylation. can be subjected acetylation by using a histone deacetylase inhibitor, so that expression amount is increased, autophagy level improved. Beclin1K416 site negative mutation adopting point technology, K416R mutant plasmid constructed. After 293T cells are transfected plasmid, partially reduced, while obvious stripe still exists. Even so, LC3 obviously inhibited.
lens.org 参考文章(3)
Sanae Shoji-Kawata, Olivier Lichtarge, Angela D. Wilkins, Beth C. Levine, Autophagy-inducing peptide ,(2013)
Tingting Li, Yipeng Du, Likun Wang, Lei Huang, Wenlin Li, Ming Lu, Xuegong Zhang, Wei-Guo Zhu, Characterization and Prediction of Lysine (K)-Acetyl-Transferase Specific Acetylation Sites Molecular & Cellular Proteomics. ,vol. 11, ,(2012) , 10.1074/MCP.M111.011080
Wang Wenxuan, Wang Yong, Bai Rui, Zhao Zhenqun, Liu Wanlin, Expression method of Beclin1 and MAP1-LC3 (microtubule-associated protein 1-light chain 3) in steroid-induced avascular necrosis of femoral head of rabbit ,(2014)