Induction of cytochrome P4501A1 and P4504A1 activities and peroxisomal proliferation by fumonisin B1

摘要: The effects of repeated exposure to fumonisin B 1 (FB ) on hepatic and renal mixed function oxidase activities peroxisomal proliferation has been examined in rats following intraperitoneal administration at three dose levels (0.125, 0.25, 2.5 mg/kg) once a day for 6 days. At the two highest doses, FB increased N-demethylation erythromycin (CYP3A1) O-deethylation ethoxyresorufin (CYP1A1). , mg/kg, also ethoxyresorufin. liver, but not kidney, was susceptible -dependent induction 12- 11-hydroxylation lauric acid, suggesting CYP4A subfamily. Immunoblot studies employing solubilized microsomes from -treated revealed that apoprotein CYP1A1 CYP4A1. same treatment with β-oxidation palmitoyl-coenzyme A (CoA) liver homogenates, immunoblot analysis showed an increase trans-2-enoyl-CoA hydratase trifunctional protein. possible implications these findings hepatocarcinogenicity this mycotoxin are discussed.