Determination of lactose by a novel third generation biosensor based on a cellobiose dehydrogenase and aryl diazonium modified single wall carbon nanotubes electrode
作者： Federico Tasca , Roland Ludwig , Lo Gorton , Riccarda Antiochia
DOI: 10.1016/J.SNB.2012.10.114
关键词: Carbon nanotube 、 Lactose 、 Nuclear chemistry 、 Cellobiose dehydrogenase 、 Biosensor 、 Detection limit 、 Glassy carbon 、 Linear range 、 Organic chemistry 、 Aryl 、 Chemistry
摘要: In this paper a new third-generation amperometric biosensor for lactose determination is described. The based on the highly efficient direct electron transfer (DET) between cellobiose dehydrogenase (CDH) from Phanerochaete sordida (PsCDH) and single walled carbon nanotubes (SWCNT). SWNCTs were surface modified with aryl diazonium salts of p-phenylenediamine (NH2-PD) deposited top glassy (GC) electrode. PsCDH NH2-PD/SWCNT-GC showed very DET exhibited an extraordinary high current density 500 mu M cm(-2) in 5 mM solution at pH 3.5. has detection limit 0.5 M, large linear range 1 to 150 sensitivity (476.8 nA M-1 cm(-2)). It shows also fast response time (4s), good reproducibility (RSD =1.75%) stability (half-life 12 days). addition, it easy, simple manufacture, cheap because low amount enzyme required selective, as no significant interference was observed. For these reasons, can represent valid alternative HPLC measurements milk dairy products. (c) 2012 Elsevier B.V. All rights reserved. (Less)
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