Resveratrol attenuates mitochondrial oxidative stress in coronary arterial endothelial cells

摘要: The production of hyperglycemia-induced mitochondrial reactive oxygen species (mtROS) is a key event in the development diabetic complications. Because resveratrol, naturally occurring polyphenol, has been reported to confer vasoprotection, improving endothelial function and preventing complications diabetes, we investigated effect resveratrol on mtROS cultured human coronary arterial cells (CAECs). measurement MitoSox fluorescence showed that attenuates both steady-state high glucose (30 mM)-induced CAECs, an was prevented by knockdown protein deacetylase silent information regulator 2/sirtuin 1 (SIRT1), intracellular target resveratrol. An overexpression SIRT1 mimicked effects attenuating production. Similar results were obtained CAECs transfected with mitochondria-targeted H2O2-sensitive HyPer-Mito fluorescent sensor. Amplex red assay significantly reduced cellular H2O2 levels as well. Resveratrol upregulated MnSOD expression increased GSH content concentration-dependent manner (measured HPLC coulometric analysis). These attenuated overexpression. We propose via pathway involves activation upregulation antioxidant defense mechanisms, production, suggesting potential for new treatment approaches targeting mitochondria metabolic diseases.