Ubiquitin profile and amyloid enhancing factor activity in Alzheimer and 'normal' human brain extracts.
作者： Z. Ali-Khan , Josee Normand , K. Alizadeh-Khiavi , Y. Robitaille , S. Chronopoulos
DOI: 10.1016/0304-3940(92)90849-3
关键词: Pathology 、 Alzheimer's disease 、 Pathogenesis 、 Amyloidosis 、 Western blot 、 Human brain 、 Dot blot 、 Molecular biology 、 Hippocampal formation 、 Biology 、 Ubiquitin
摘要: Abstract Tris-HCl or Laemmli sample buffer extracted frontal lobe and hippocampal samples from normal aged Alzheimer's disease (AD) subjects were used to determine total ubiquitin (Ub), distribution of monomeric Ub Ub-protein conjugates amyloid enhancing factor (AEF) activity using the dot-blot. Western blot mouse AEF bioassay techniques, respectively. The AD samples, as compared normals, demonstrated a 1.7-fold increase in Ub, elevated levels an appreciably enhanced activity. Many found be soluble only buffer. possible roles association with are discussed regard pathogenesis brain amyloidosis.
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