In situ hybridization with digoxigenin-labeled RNA probes: facts and artifacts.

摘要: We have developed an easy, stream-lined yet sensitive protocol for in situ hybridization to mRNA frozen tissue sections or cytospins using digoxigenin-labeled RNA probes detected by alkaline phosphatase. found the crucial parameters successfully performing this technique be quality, fixation time and effective removal of excess probe. Most preparation, incubation steps washes as described literature were unnecessary and, therefore, eliminated, making simple enough achieved any laboratory with access preparation some molecular biology expertise.